Quick Answer: What Is The Difference Between Differential And Selective Media?

Why is selective and differential media important?

Selective and differential media are used to isolate or identify particular organisms.

Selective media allow certain types of organisms to grow, and inhibit the growth of other organisms.

Differential media are used to differentiate closely related organisms or groups of organisms..

How is EMB Agar selective and differential?

Eosin Methylene Blue (or EMB) Agar is a Selective & Differential Medium. The selective and differential aspects are due to the dyes Eosin Y and Methylene Blue, and the sugars lactose and sucrose in the medium. It is Selective because it encourages some bacteria to grow while inhibiting others.

Which of the following media is both selective and differential?

Another commonly used medium that is both selective and differential is eosin-methylene blue (EMB) agar. EMB contains the dyes eosin and methylene blue that inhibit the growth of gram-positve bacteria. Therefore, EMB is selective for gram-negatives.

What is an example of differential media?

Examples of differential media include: Blood agar (used in strep tests), which contains bovine heart blood that becomes transparent in the presence of hemolytic. … MacConkey (MCK), which is differential for lactose fermentationmannitol salt agar (MSA), which is differential for mannitol fermentation.

Is pea selective or differential?

PEA agar is a selective medium that permits the growth of gram-positive cocci while inhibiting most gram-negative organisms.

Why is MacConkey agar selective as well as differential?

Why is MacConkey agar selective as well as differential? It’s selective to gram negative enteric bacteria because it contains bile salts, which inhibit most gram positive bacteria. It also contains lactose, which differentiates between gram negative lactose fermenter and gram negative non-fermenters.

Is starch agar selective or differential?

Starch agar is a differential medium that tests the ability of an organism to produce certain exoenzymes, including a-amylase and oligo-1,6-glucosidase, that hydrolyze starch.

What media is used for E coli?

Choose your type of bacterial growth media:LB Broth and LB AgarMost referenced bacterial media for growth and maintenance of recombinant E. coli strainsTerrific BrothNutritionally rich bacterial media for higher density growth and maintenance of recombinant E. coli strains4 more rows

What is the definition of selective media?

What is the definition of selective media? Selective media incorporate chemical substances that inhibit the growth of one type of bacteria while permitting the growth of another. … Differential media incorporate chemical compounds that produce characteristic changes in the appearance of the bacterial colonies.

What is the purpose of peptone in the media?

What is the purpose of peptone in the media? It provides nutrients like carbon and nitrogen to bacteria. What is the purpose of agar in the media? It is a solidifying agent.

What is a differential media?

Differential media contain compounds that allow groups of microorganisms to be visually distinguished by the appearance of the colony or the surrounding media, usually on the basis of some biochemical difference between the two groups.

What is an example of selective media?

Examples of selective media include: Eosin methylene blue contains dyes that are toxic for Gram-positive bacteria. … MacConkey agar is for Gram-negative bacteria. Hektoen enteric agar is selective for Gram-negative bacteria.

Is MacConkey agar selective or differential?

MacConkey agar is a selective and differentiating agar that only grows gram-negative bacterial species; it can further differentiate the gram-negative organisms based on their lactose metabolism.

What are the 3 main types of microbiological culture media?

These are classified into six types: (1) Basal media, (2) Enriched media, (3) Selective media, (4) Indicator media, (5) Transport media, and (6) Storage media. 1. BASAL MEDIA. Basal media are those that may be used for growth (culture) of bacteria that do not need enrichment of the media.

What bacteria can grow on blood agar?

Blood Agar is used to grow a wide range of pathogens particularly those that are more difficult to grow such as Haemophilus influenzae, Streptococcus pneumoniae and Neisseria species. It is also required to detect and differentiate haemolytic bacteria, especially Streptococcus species.

What is the use of nutrient agar?

Nutrient agar is a general purpose medium supporting growth of a wide range of non-fastidious organisms. It typically contains (mass/volume): 0.5% Peptone – this provides organic nitrogen. 0.3% beef extract/yeast extract – the water-soluble content of these contribute vitamins, carbohydrates, nitrogen, and salts.

What bacteria does not grow on blood agar?

Blood agar is an enriched, bacterial growth medium. Fastidious organisms, such as streptococci, do not grow well on ordinary growth media. Blood agar is a type of growth medium (trypticase soy agar enriched with 5% sheep blood) that encourages the growth of bacteria, such as streptococci, that otherwise wouldn’t grow.

What is the difference between selective and differential media quizlet?

Differential media helps you distinguish between groups of organisms that are related. You can often tell the difference by color or colonies. What is the purpose of selective media? The purpose of selective media is to isolate specific types of bacteria.

Is blood agar selective or differential?

Blood Agar This medium can be made selective by the addition of antibiotics. This medium is differential because: Blood Agar tests for the production of hemolysin which breaks down red blood cells.

Is chocolate agar selective or differential?

Chocolate agar, a differential medium for gram-positive cocci.